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Immunology Effects of Anti-atherosclerosis Protein Vaccines Based on Heat Shock Protein 65 |
SUN Yun-xiao1,2, LU Yong1, LI Zhu-fang1, XU Qiang2, LIU Xiao-ming2, LI Tai-ming1, LIU Jing-jing1 |
1. Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;
2. Research and Development Center of Nonhuman Primates, South China Institute of Endangered Animals, Guangzhou 510260, China |
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Abstract By using two functional epitopes of HSP65 with preventive effects to autoimmune inflammatory disease, high-level expression vectors of pET28a-HSP65-P1P2P1 and pET28a-CTB-P1P2P1 were built with HSP65 and CTB as a carrier protein respectively. The fusion protein of HSP65-P1P2P1 and CTB-P1P2P1 were acquired by anion exchange column chromatography and then used to immunize the New Zealand white rabbits with atherosclerosis disease induced by high cholesterol diet-fed. The animals were nasally immunized with low-dose proteins for multiple times. The results showed that two groups of fusion proteins possessed the effectiveness of reducing atherosclerotic plaque formation. Compared with the PBS control group the aortic lesion areas of the two groups decreased by 34.9% and 27.9% separately. The study provided a good design idea for the further development of clinical anti-AS vaccine. After further optimization design for increasing their immunogenicity, it could be developed as a vaccine against atherosclerosis.
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Received: 11 August 2010
Published: 25 January 2011
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