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| Very High Gravity Ethanol Continuous Fermentation in Reaction System Composed by Packing Tubular Reactors |
| SHEN Yu1,2, BAI Feng-wu2 |
1. Engineering Research Centre for Waste Oil Recovery Technology and Equipment, Ministry of Education, Chongqing Technology and Business University, Chongqing 400067, China;
2. College of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116023, China |
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Abstract A fermentation system composed of a stirred tank followed by four-stage packed tubular bioreactors in series was set up to evaluate very high gravity ethanol continuous fermentation using Saccharomyces cerevisiae. Polyurethane and wood chips were used for cells immobilization in tubular reactors, and ethanol productivity in later reactors were maintained at high level by high bilomass content even the viability and ethanol productivity of cells were inhibited by high ethanol content. The system ran at 0.02h-1 and fermented very high gravity medium containing 280 g/L glucose to produced 15.4 % v/v ethanol. It was also proved that dilution rat should reduce by new reactors added to higher final ethanol content under the lower dilution rat level.
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Received: 09 October 2010
Published: 01 April 2011
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