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The Effect of Aeration on the Growth and Lipid Content on Chlorella vulgaris LICME002 in the Applied Alumina Gas Distributor Bubbling Column Photobioreactor |
ZHANG Qi1, GAO Zhen2, HUANG He1,2, LIANG Xi-hai1, JI Xiao-jun1, ZHENG Hong-li1, YIN Feng-wei1 |
1. Nanjing University of Technology, Jiangsu 210009, China;
2. State Key Laboratory of Materials-Oriented Chemical Engineering, Jiangsu 210009, China |
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Abstract In the study of photobioreactor, gas distributor has great influence to the growth of microalgae, especially in the bubbling column reactor.The effect of the gas-flow rate and CO2 concentration on the biomass, chlorophyll a, and lipid accumulation of Chlorella vulgaris LICME002 in the 5L bubbling photobioreactor with a alumina gas distributor. The results showed that the 3% CO2 is the optimum condition for biomass, chlorophyll a, oil accumulation. When the CO2 concentration exceeded 6%, the algae's parameters decreased significantly. With the analysis of the algae's parameters at 0.1vvm,0.4vvm,0.7vvm, 1.0vvm, and the gas-flow rate 0.4vvm is the best one. Results showed that the optimum gas-flow rate and CO2 concentration, the microalgae biomass can achieve 1.52 g/L, oil content achieved 31.5%.
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Received: 14 October 2010
Published: 01 April 2011
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[1] Degen J, Uebele A, Retze A, et al. A novel airlift photobioreactor with baffles for improved light utilization through the flashing light effect. Biotechnol,2001, 92(2):89-94.
[2] Barbosa J, Janssen M, Ham N, et al. Microalgae cultivation in air-lift reactors: modeling biomass yield and growth rate as a function of mixing frequency. Biotechnol Bioeng,2003, 82(2):170-179.
[3] Vega-Estrada J, Montes-Horcasitas M C, Domínguez-Bocanegra AR, et al. Haematococcus pluvialis cultivation in split-cylinder internal-loop airlift. photobioreactor under aeration conditions avoiding cell damage. Appl Microbiol Biotechnol,2005, 68(1):31-35.
[4] Buwa V V, Ranade V V. Dynamics of gas-liquid flow in a rectangular bubble column: experiments and single/multi-group CFD simulations. Chem Eng,2002, 57(22-23):4715-4736.
[5] Buwa V V, Ranade V V. Characterization of dynamics of gas-liquid flows in rectangular bubble columns. A.I.Ch.E. J, 2003,12(50):2394-2407.
[6] Abraham M, Sawant S B. Effect of sparger design on the hydrodynamics and mass transfer characteristics of a bubble column. Indian Chem Eng, 1989,1:31-38.
[7] Li G, Yang X G, Dai G. CFD simulation of effects of the configuration of gas distributors on gas-liquid flow and mixing in a bubble column. Chemical Engineering Science,2009, 64(24):5104-5116.
[8] Li J M, Cheng L H, Xu X H. Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. Bioresource Technology,2010, 101(17):6797-6804.
[9] Takagi M, Karseno, Yoshida T. Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella Cells. J Biosci Bioeng, 2006, 101(3):223-226.
[10] Chiu S Y, et al. Reduction of CO2 by a high-density culture of Chlorella sp.in a semicontinuous photobioreactor. Bioresource Technology,2008, 99(9):3389-3396.
[11] Hu H, Gao K. Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources.Biotechnol Lett,2003, 25(5):421-425.
[12] Watanabe Y, Ohmura N, Saiki H. Isolation and determination of cultural characteristics of microalgae which functions under CO2 enriched atmosphere. Energy Conversion Management,1992, 33 (5-8):545-552.
[13] Pronina N A, Kodama M, Miyachi S. Changes in intracellular pH values in various microalgae induced by raising CO2 concentrations. XV Int Botanical Cong,1993:419.
[14] Pulles Martin P J, Hans J. van Gorkoma, et al. Verschoora Primary reactions of Photosystem II at low pH. 2. Light-induced changes of absorbance and electron spin resonance in spinach chloroplasts. Biochimica et Biophysica Acta - Bioenergetics,1976, 1(440):98-106.
[15] Iwasaki I, Kurano N, Iwasaki I S M, et al. Effects of high-CO2 stress on photosystem II in a green alga, Chlorococcum littorale, which has a tolerance to high CO2.Journal of Photochemistry and Photobiology,1996, 3(36):327-332.
[16] Kaplan A, Reinhold L. CO2 concentrating mechanisms in photosynthetic microorganisms. Annu Rev Plant Physiol Plant Mol Biol, 1999,50:539-570.
[17] Giordano M, Beardall J, Raven J A. CO2 concentrating mechanisms in algae: mechanisms, environmental modulation, and evolution. Annu Rev Plant Biol, 2005, 56:99-131.
[18] Lee J S, Shin C S, Park S C. CO2 fixation by Chlorella sp. KR-1 and its cultural characteristics. Bioresource Technol, 1999, 68(3):269-273.
[19] Zhang Y H, Yang S S. Some characteristics of microalgae isolated in Taiwan for biofixation of carbon dioxide. Bot Bull Acad Sinca, 2003, 44(1):43-52
[20] de Morais M G, Costa J A V. Biofixation of carbon dioxide by Spirulina sp and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor. J. Biotechnol, 2007, 129(3):439-445.
[21] de Morais M G, Costa J A V. Isolation and selection of microalgae from coal fired thermoelectric power plant for biofixation of carbon dioxide. Energy Conv Manag,2007, 48(7):2169-2173.
[22] Livne A, Sukenik A . Lipid Synthesis and A bundance of Acetyl CoA Carboxylase in Isochrysis galbana (Prymnesiophyceae) Following Nitrogen Starvation. Plant Cell Physiol,1992, 33(8):1175-1181.
[23] Sasaki Y, Nagano Y. Plant acetyl-CoA carboxylase: structure, biosynthesis, regulation, and genemanipulation for plant breeding. Bioscience, Biotechnology and Biochemistry,2004, 68 (6):1175-1184.
[24] Sukenik A, Line A.Variations in lipid and fatty acid content in relation to acetyl CoA carboxylase in the marine prymnesiophyte Isochrysis galbana. Plant Cell Physiol,1991, 32(3):371-378.
[25] Kinney A J. Genetic modification of the storage lipids of plants. Curr Opin Biotechnol,1994, 5(2):144-151.
[26] Falkowski P G, Raven J A. Aquatic photosynthesis. London: Blackwater Science,1997.375.
[27] Zilinskas B G, Zilinskas B B. Light absorption, emission and photosynthesis. Algal physiology and biochemistry. Oxford: Blackwell Scientiic Publications,1974.346-390.
[28] Prokop A, Erickson L E. Photobioreactors. In: Prokop A, Erickson L E, editiors. Bioreactor system design. New York: Marcel Dekker, Inc,1995.441-477.
[29] Merchuk J C. Shear Effects on Suspended Cells . Adv Biochem Eng,1991, 44: 65-95.
[30] Michels M H A, van der Goot A J, Norsker N H, et al. Effects of shear stress on the microalgae Chaetoceros muelleri. Bioprocess Biosyst Eng,2010, 33(8):921-927.
[31] Csordas A, Wang J K . An integrated photobioreact or and foam fractionation unit for the growth and harvest of Chaetoceros spp. in open systems. Aquacultural Engineering,2004, 30(1-2):15-30. |
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