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| The Inhibitory Effect of Arresten Protein on Angiogesis |
| LV Yi, ZHENG Jin-ping |
| Department of Health Toxicology, Shanxi Medical University, Taiyuan 030001, China |
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Abstract Objective:To investigate the effect of Arresten protein on proliferation and migration of human umbilical vein endothelial cell (HUVEC) and the inhibitory effect of Arresten protein on angiogenesis. Methods:Arresten protein was incubated with HUVEC in vitro: The effect on proliferation of HUVEC was examined using MTT assay. The effect on apoptosis of HUVEC was monitored by FCM. The effect on migration of HUVEC was examined by Boyden Chamber. Chicken chorioallantoic membrane (CAM) angiogenesis model was used to identify the influence of Arresten protein on neovascularization. Results:Arresten protein inhibits the proliferation and migration of HUVEC and promotes its apoptosis effectively; And the effect was dose-dependent. Compared with PBS group, the vessels of Chicken chorioallantoic membrane (CAM) in Arresten protein treated groups were obviously damaged(P<0.01). So Arresten protein can significantly inhibit angiogenesis of CAM. Conclusion:Arresten protein inhibits angiogenesis through the inhibition on HUVEC.
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Received: 09 September 2010
Published: 25 January 2011
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