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| Expression and Purification of Disulfide-rich Peptide Toxins |
| ZHANG Ke-jun1,2, LI Li1, DAI Qiu-yun2 |
1. College of Life Science, Shaanxi Normal University, Xi'an 710062, China;
2. Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China |
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Abstract The disulfide-rich peptide toxins possess low molecular weight, tight disulfide bridges and low folding efficiency in vitro, hence they are difficult to be chemically synthesized efficiently when sequences are more than 40 amino acids. This review briefly summarizes the progress of genetic expression of disulfide-rich peptide toxins (such as conotoxins, scorpion and spider toxins), especially focuses on the technical features of their expressions and purifications in Escherichia coli and yeast.
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Received: 08 July 2013
Published: 25 November 2013
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