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| Study on Recombination and Preparation of PACAP-derived Polypeptide RHMP and Its Effects on Promoting Corneal Wound Healing |
| XU Wen-na, MA Yi, YE Zu-lu, LUO Tian-jie, RAO Lei, HONG An |
| Department of Cell Biology of Jinan University, Institute of Biological Medicine of Jinan University, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Guangzhou 510632, China |
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Abstract Preparing pituitary adenylate cyclase-activating polypeptide (PACAP) recombinant peptide RHMP using genetic engineering techniques and exploring its biological effects on corneal wound healing in vitro can provide novel candidate drug and method for the treatment of corneal injury and research. Using genetic recombinant technology to prepare recombinant peptide, expressed fusion protein were purified with Chitin-Beads columns, the released polypeptide were analyzed and identified with HPLC, SDS-PAGE and MS, then studied on its effects on corneal wound healing in mice. Experimental results showed that the molecular weight of RHMP is 3.4 kDa, its purity is 96%. In addition, the corneal repair rates of experimental group after injury are higher than control, the difference is very significant. Therefore, realized efficient synthesis of RHMP, and it can significantly promote corneal wound healing in mice, so it will likely to become a new drug for corneal injury. At the same time the corneal epithelial wound model in mice can be effectively used to study on the biological effects of the related drugs.
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Received: 04 September 2012
Published: 25 February 2013
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