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| Asymmetric Reduction of 2-Octanone with the Yeast Carried on Macromolecule Membrane |
| XIONG Long-bin, ZENG Hong-yan, HE Ping, TAN Yi-zhe, LIU Xue-ying, ZHANG Cun-ying, CAI Xi-ling |
| College of Chemical Engineering, Biotechnology Institute, Xiangtan University, Xiangtan 411105, China |
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Abstract The baker yeast DX213 (Saccharomyces cerevisiae DX213) was immobilized on the activated woven nylon-6 membrane using glutaraldehyde as a cross-linking agent. In the process of asymmetric reduction of 2-octanone to 2-octanol by the immobilized yeast cells, the effect of organic solvent, reaction time, pH, 2-octanone, co-substrate and heating treatment on the yield and enantiomeric excess(e.e. value) of (R)-2-octanol was studied. The function mechanism of the influence factors with enzymatic activity of the cells was presented in the reaction. The results showed that all the mentioned factors had effects on the reaction. Dichloromethane was found to be the optimal organic solvent for the reaction. Under the conditions of 57 g/L immobilized cell preheated at 50℃ for 50 min, 4/1 volume ratio of water phase to organic phase, pH7.0, 60 mmoL/L initial 2-octanone concentration added in three separate doses at 0, 10 and 27 h, 5.7 g/L sucrose concentration, 28℃ and reaction time 48 h, the yield and enantiomeric excess of (R)-2-octanol were 89.3 % and 96.8%, respectively.
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Received: 20 August 2012
Published: 25 February 2013
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