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| Optimization of Fermentation in Shake Flasks for the Xylanase in Recombinant E. coli |
| HE Jie1,2, SU Ling-qia1,2, WU Jing1,2 |
1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2. School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, Wuxi 214122, China |
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Abstract In order to achieve high production of the xylanase, the optimization of culture medium was investigated in E. coli BL21(DE3) harboring the plasmid pET20b(+)/coe/xynA. The optimized medium and induction condition were as follows: 11 g/L glycerol, 24 g/L yeast extract, 8 g/L peptone, 89 mmol/L PO43-, 4 mmol/L Mg2+, induced by 0.4 mmol/L IPTG at 6h of culture. Under this condition, the enzyme activity increased from 346.8 U/ml to 780.2 U/ml, which was 2.2 times as high as that when it was not optimized.
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Received: 09 December 2012
Published: 25 February 2013
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