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| Effects of High Magneto-Gravitational Environment on Pseudomonas aeruginosa N1207 |
| ZHAO Jian-feng1, XIN Xing1, WEI Pei-pei1, QIAN Ai-rong2, Akateh Tazifua Alfred1, SHANG Peng2, YANG Shu-lin1 |
1. School of Environment & Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, China;
2. Key Laboratory for Space Biosciences & Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China |
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Abstract The aim is to investigate the effects of a High Magneto-Gravitational Environment (HMGE) produced by a superconducting magnet on Pseudomonas aeruginosa N1207. A specially designed large gradient superconducting magnet was used to produce gravity levels from hypo-gravity (0 g) to hyper-gravity (2 g) and relevant magnetic field strengths, namely (0 g, 12 T), (1 g, 16 T) and (2 g, 12 T). After Pseudomonas aeruginosa N1207 was mutated in HMGE for 24h, 48h, 72h, respectively, the mutant M14808 with the highest yield of rhamnolipids was isolated from the strongest magnetic field (16 T). Results showed that the rhamnolipid production of M14808 was dramatically increased by over 30%, and maintained a constant yield for 6 generations. Comparing the growth cycle of the original strain and mutants, it was observed that mutants reached the exponential phase earlier than the original strain. The mutation result indicated that the high magnetic field was primarily responsible for the production of rhamnolipids and the growth rate. The antitumor cytotoxicity experiment showed that crude di-rhamnolipids could inhibit the growing of four cancer cell lines, MCF-7, H460, HepG2 and A549, with the IC50 of di-rhamnolipids to MCF-7 as low as 125.13μg/ml.
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Received: 11 December 2012
Published: 25 February 2013
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| Fund: This work was supported by the National "863" High Technologies Research Foundation of China (No.2088AA12A218-12) |
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