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| Advances on Expression of Snake Venom Protein In Pichia pastoris |
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Abstract Abstract Snake venom is a mixture of many proteins and enzymes with distinct biological activities, which may have significant values both in basic researches and clinical applications. However, limitation exists in the purification of sufficient active fractions for practical use. Pichia pastoris is the most commonly used eukaryotic expression system nowadays. The ability of eukaryotic processing, folding and post-translational modification endow the recombinant proteins expressed with similar biological activities of the corresponding natural proteins, is thus widely adopted to express snake venom proteins with glycosylation or rich in disulfide bonds. To date, about twenty-five snake venom proteins of 12 genus have been expressed successfully in Pichia pastoris, (including different snake venom family members such as serine proteinases, metalloproteinases/disintegrins, L-amino acid oxidases, C-type lectin like proteins, neurotoxins, vasoconstrictors, nerve growth factor,etc.),so far,snake venom cysteine-rich protein, Bradykinin-potentiating peptides ect. expressed in Pichia pastoris have not been reported. The reasons for the failure of expression of snake venom protein in Pichia pastoris maybe as follows: the codon bias, RNA secondary structure of the gene transcripted, heterogeneity of glycosylation and the difference between glycosylation types, the toxicity of expressed toxic protein on yeast cells ect.,we also discussed the measures to these problems in this paper.
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Received: 19 May 2010
Published: 19 October 2010
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| Fund: ;The natural Science Foundation of Shaanxi Province; the Fundamental Research Funds for the Central Universities |
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