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| Enhancement of Transglutaminase Production in Streptoverticillium mobaraensis as Achieved by Treatment with NaCl |
| CHEN Jia1, JIN Ming-fei1, TAN Yu-jing2, LIU Ying1, TIAN Pei-lin1, CHANG Zhong-yi1, BU Jian-guo3 |
1. Life Science College of East China Normal University, Shanghai 200241, China;
2. Shanghai Institute of Fibre Inspection, Shanghai 200240, China;
3. Taixing Dongsheng Food Science and Technology Limited Company, Taixing 225411, China |
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Abstract The effects of three inorganic salts on the production of transglutaminase (TGase) in Streptoverticillium mobaraense were tested. It indicated that enzyme activity increased in the presence of 0.5% MgCl2 or 0.5% NaCl compared with control, and 0.5% NaCl functioned better. The SDS-PAGE results showed that the total amount of pro-TGase and TGase in each sample points of experimental group was more than that of control group, and the increase of TGase of experimental group was faster than control. So NaCl had a significant effect on the TGase production by promoting the secretion of proenzyme (pro-TGase) and the maturation of TGase. Furthermore, the optimum addition of NaCl was 0.5% so that the TGase activity increased by more than 12%, and the fermentative period shortened 15 hours.
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Received: 20 August 2012
Published: 25 February 2013
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