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| Effects of RUNX2 on BMP9-induced Osteogenic Differentiation of C3H10T1/2 Mesenchymal Stem Cells |
| ZHAO Yan-fang, SONG Tao, LIU Yue-liang, LUO Jin-yong |
| Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective: To investigate and validate the effects of RUNX2 on BMP9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells.Methods: Expression level of RUNX2 was measured by luciferase activity assay, RT-PCR and Western blot, and the effects of RUNX2 on BMP9-induced osteogenic differentiation of C3H10T1/2 were detected by Alkaline phosphatase staining, Alizarin red S staining, immunocytochemical and entopic bone formation.Results: BMP9 increased the expression level of RUNX2.In vitro,RUNX2 increased BMP9-induced early osteogenic marker ALP activity and late osteogenic marker calcium deposition, however, inhibited OCN expression.In vivo, RUNX2 enhanced BMP9-induced entopic bone formation.Moreover,RUNX2 gene silence was showed to inhibit ALP activity,calcium deposition and entopic bone formation.Conclusion: In a word,RUNX2 may promote BMP9-induced osteogenic differentiation of C3H10T1/2.
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Received: 13 June 2012
Published: 25 February 2013
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