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| Effects of Humoral Immune Response Vaccinated by Sequential Inactivated-live Rotavirus Vaccine |
| ZHANG Biao1,3, TONG Lin2, YI Shan3, ZHANG Guang-ming3, LI Hong-jun3, SUN Mao-sheng3, CHEN Dong1 |
1. Department of Histology and Embryology, Guangdong Medical College, Zhanjiang 524023, China;
2. Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China;
3. Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Science, Peking Union Medical College, Kunming 650118, China |
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Abstract Objective: To evaluate the humoral immune responses vaccinated by sequential inactivated-live rotavirus vaccines, primary immune with inactivated rotavirus vaccine (IRV) and boost immune with live rotavirus vaccine. Methods: The mice were randomly divided into four groups (oral vaccine group, sequential vaccine group, oral control group and sequential control group) and immunized by respective protocols. Then, the levels of serum rotavirus specific IgG, IgA and fecal rotavirus specific IgA were assessed by ELISA, the levels of serum neutralizing antibody were measured by microneutralization assay, the rotavirus sheddings in fecal following live rotavirus vaccine vaccination were assessed by ELISA. Results: Compared to sequential control groups, the levels of serum IgG, IgA, neutralizing antibody and fecal IgA were higher in sequential vaccine groups. Compared to oral vaccine groups, sequential vaccination induced the higher levels of serum IgG, IgA, neutralizing antibody, but there was no significant difference for the levels of fecal IgA between two groups. At the same time, it was found that there were no significant differences for the amounts and time of rotavirus shedding after first vaccination of live rotavirus vaccine between sequential vaccine groups and oral vaccine groups. However, after second vaccination of live rotavirus vaccine in sequential vaccine groups, the amounts and time of rotavirus shedding rapidly decrease, which were similar to the rotavirus shedding after third vaccination of live rotavirus vaccine in live vaccine groups. Conclusion: The systemic and mucosal humoral immune responses were induced by sequential inactivated-live rotavirus vaccines in mice. The protocol of vaccination with sequential inactivated-live rotavirus vaccines may be used as a candidate protocol for clinic application of rotavirus vaccine in the future.
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Received: 10 August 2012
Published: 25 February 2013
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