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| Construction of Bovine Lactoferricine Transgenic Mice |
| ZHANG Jin-xia, FAN Zhi-qiang, LIU Ye, ZHANG Shou-feng, YANG Yang, WANG Ying, ZHANG Fei, HU Rong-liang |
| Veterinary Institute, Academy of Military Medical Sciences, Changchun 130122, China |
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Abstract Purpose: To investigate the expression of bovine lactoferricine in mammary gland of the transgenic mice and the antibacterial activity. Methods: Mammary gland expression vector PI-bcp-LfcinB containing the goat β-casein promoter and bovine lactoferricine gene was digesting with Xho I and Nru I. DNA fragment containing the expression elements was gained for microinjection. About 500 fertilized eggs of mice were injected using classic microinjection technique,and about 430 living eggs were transplanted into 13 pseudopregnant mice,among which 6 pregnant mice produced 13 offspring.These offspring were identified by PCR,1 mice was bovine lacteferricine transgenic mice.Target gene can be transferred by the transgenic mice,which was proved by PCR. The expression of bovine lactoferricine of transgene mice was detected by RT-PCR and Kirby-Bauer. Results: These tests showed that the mammary-tissue-specific expression vector driven by the goat beta-casein gene promoter could efficiently direct the expression of bovine lactoferricine in mice milk. And the expressed product exhibited evident bactericidal activity to Escherichia coli. Conclusion: It is feasible to get bioactive bovine lactoferricine through the mammary gland of animal and it provides a basis for disease-resistant animal breeding and production of antibacterial peptides through transgenic animals.
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Received: 16 October 2012
Published: 25 February 2013
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