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| Zinc Finger Nucleases and Targeted Genome Engineering in Plants |
| SONG Yun, QIAO Yong-gang, LI Gui-quan |
| College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China |
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Abstract Zinc finger nucleases (ZFNs) typically consist of three to four zinc fingers (ZFs) and the non-specific DNA-cleavage domain of the restriction endonuclease FokⅠ. In this configuration, the ZFs constitute the binding module and the FokⅠ domain the cleavage module. ZFNs offer a general way to deliver a targeted site-specific double strand break (DSB) to the genome. DSBs are mainly repaired by two pathways, non-homologous end joining (NHEJ) and homologous recombination (HR), which targeted mutagenesis will be generated. In this review, we are first describe the structure of ZFNs, and ZFN-mediated targeted genome engineering via HR and NHEJ,and the methods of construct novel ZFNs and the steps of ZFN-mediated gene targeting. Then we mainly explore that ZFNs were used in plants.
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Received: 11 July 2012
Published: 25 January 2013
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