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| Medium Optimization for the Production of New Antifungl Cyclic Lipopeptide Marinhysin A by Bacillus Marinus B-9987 |
| CHEN Jie1, WEI Hong-gang1, LUO Yuan-chan1, ZHANG Dao-jing1, LI Shu-lan2, TIAN Li3, LI Yuan-guang1 |
1. East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, Shanghai 200237, China;
2. Shanghai Zeyuan Marine Biotechnology Co., Ltd., Shanghai 200237, China;
3. The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China |
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Abstract The new antifungal cyclic lipopeptide Marinhysin (MA) produced by Bacillus marinus B-9987 shows the excellent activity against plant pathogens both in vitro and in vivo. In the present study, the response surface methodology (RSM) was employed to optimize the medium composition for producing MA. The results of the Plackett-Burman design showed that sucrose and yeast powder had significant effects on MA production. The central composite design was applied to further optimize the concentration of sucrose and yeast powder in the medium. The results of analysis suggested that the optimum values of the tested variables were sucrose of 42.0 g/L and yeast powder of 42.3 g/L. MA concentration reached 75.74 mg/L, which was in agreement with the predicted value of 68.19mg/L. In comparison to the production of original level (54.12mg/L) in a 250ml flask, 1.28-fold increment had been obtained. By scaling u Pthe fermentation from flask to a 5L fermentor with the optimal medium, MA concentration was increased to 182mg/L, which was 1.28 times of the value (130mg/L) with the original medium.
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Received: 01 November 2012
Published: 25 January 2013
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