|
|
ε-Poly-L-lysine Production from Precursor L-lysine by Streptomyces sp. M-Z18 |
CHEN Xu-sheng, REN Xi-dong, ZENG Xin, DONG Nan, MAO Zhong-gui |
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
|
|
Abstract To investigate the process of ε-poly-L-lysine (ε-PL) production from precursor L-lysine under different culture conditions of Streptomyces sp. M-Z18, it has developed conversion of precursor L-lysine for ε-PL production, combined with two-stage culture method, and with fermentation from glycerol, respectively. The results of experiment showed that two-stage culture method was used for conversion L-lysine to ε-PL and attained at 15 g/L with biotransformation of 3 g/L L-lysine; Furthermore, ε-PL production from glycerol fermentation coupled with L-lysine conversion achieved 33.76 g/L ε-PL and enhanced ε-PL productivity at 37.8%, compared with L-lysine-free fermentation. It was demonstrated that ε-PL production could be derived from precursor L-lysine, however, the efficiency of this conversion is needed further improved. It has indicated that the limit of ε-PL production is the biosynthesis of L-lysine in primary metabolism. Meanwhile, the results presented here provide a guidance for the ε-PL-producing strains primary metabolism improvement by metabolic engineering, and an efficient approach to enhancement of ε-PL production in scale fermentation.
|
Received: 19 September 2012
Published: 25 January 2013
|
|
|
|
[1] Hiraki J, Ichikawa T, Ninomiya S, et al. Use of ADME studies to confirm the safety of ε-polylysine as a preservative in food. Regul Toxicol Pharm, 2003, 37:328-340. [2] Hiraki J, Morita H. Strain mass-producing ε-poly-L-lysine, a method for using its strain and a method for producing ε-poly-L-lysine. European Patent, EP0256423A2.1988. [3] Hiraki J, Hatakeyama M, Morita H, et al. Improved ε-poly-L-lysine production of an S-(2-aminoethyl)-L-cysteine resistant mutant of Streptomyces albulus. Seibutu Kougaku Kaishi, 1998, 76:487-493. [4] Kahar P, Iwata T, Hiraki J, et al. Enhancement of ε-polylysine production by Streptomyces albulus strain 410 using pH control. J Biosci Bioeng, 2001, 91:190-194. [5] Hiraki J, Suzuki E. Process for producing ε-poly-L-lysine with immobilized Streptomyces albulus. US Patent. US 5900363.1996. [6] Liu S G, Wu Q P, Zhang J M, et al. Production of ε-poly-L-lysine by Streptomyces sp. using resin-based, in situ product removal. Biotechnol Lett, 2011, 33:1581-1585. [7] Chen X S, Ren X D, Dong N, et al. Culture medium containing glucose and glycerol as a mixed carbon source improves ε-poly-L-lysine production by Streptomyces sp. M-Z18. Bioprocess Biosyst Eng, 2012, 35:469-475. [8] Shima S, Oshima S, Sakai H. Biosynthesis of ε-poly-L-lysine by washed mycelium of Streptomyces albulus No.346. Nippon Nogeikagaku Kaishi, 1983, 57:221-226. [9] Hirohara H, Takehara M, Saimura M, et al. Biosynthesis of poly(ε-L-lysine)s in two newly isolated strains of Streptomyces sp. Appl Microbiol Biotechnol, 2006, 73:321-331. [10] 谢志鹏, 徐志南, 郑建明, 等. 靛酚蓝反应测定发酵液中的氨态氮. 浙江大学学报(工学版), 2005, 39:437-444. Xie ZH P, Xu ZH N, Zheng J M, et al. Determination of ammonium nitrogen in fermentation broth through indophenol blue reaction. Journal of Zhejiang University (Engineering Science), 2005, 39:437-444. [11] Itzhaki R F. Colorimetric method for estimating polylysine and polyarginine. Anal Biochem, 1972, 50:569-574. [12] Chen X S, Li S, Liao L J, et al. Production of ε-poly-L-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol. Bioprocess Biosyst Eng, 2011, 34:561-567. [13] 郭明,胡昌华. 生物转化—从全细胞催化到代谢工程. 中国生物工程杂志, 2010,30:110-115. Guo M, Hu CH H. Bioconversion from whole cell biocatalysis to metabolic engineering. China Biotechnology, 2010, 30:110-115. [14] 张琪,王秀伶,王世英,等. 牛瘤胃分离菌株静息细胞培养体系生物转化黄豆苷原. 生物工程学报, 2010, 26: 35-41. Zhang Q, Wang X L, Wang SH Y, et al. Biotransformation of daidzein by resting cell system of bacterial strain isolated from bovine rumen gastric juice. Chinese Journal of Biotechnology, 2010, 26: 35-41. [15] Yamanaka K, Kito N, Imokawa Y, et al. Mechanism of epsilon-poly-L-lysine production and accumulation revealed by identification and analysis of an epsilon-poly-L-lysine-degrading enzyme. Appl Environ Microbiol, 2010,76: 5669-5675. [16] Liang G B, Du G C, Chen J. A novel strategy of enhanced glutathione production in high cell density cultivation of Candida utilis—Cysteine addition combined with dissolved oxygen controlling. Enzyme Microb Technol, 2008, 42:284-289. [17] Yoon S H, Do J H, Lee S Y, et al. Production of poly-γ -glutamic acid by fed-batch culture of Bacillus licheniformis. Biotechnol Lett, 2000, 22:585-588. [18] 贾士儒,莫治文,谭之磊,等. 一种提高ε-聚-L-赖氨酸的新方法. 中国专利,CN101671703A.2010. Jia SH R, Mo ZH W, Tang ZH L, et al. A new method for improving ε-poly-L-lysine production. China Patent, CN101671703A.2010. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|