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Isolation of a Fungal Strain of Degrading BTEX and Its Degradation Characteristics |
ZHANG Zhan-sheng1, LI Yan-xu1, DU Qing-ping1, XIA Li-juan2 |
1. School of Environmental Science and Engineering, Guangdong University of Technology,Guangzhou 510006, China; 2. School of Chemical Engineering and Light Industry, Guangdong University of Technology,Guangzhou 510006, China |
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Abstract By gradually increasing the concentration of BTEX in the culture medium with toluene, benzene and xylene as the sole carbon source, a BTEX-degrading fungi strain which was designated as B1 was isolated from the sludge of sewage treatment plant. Using single factor and orthogonal experiment analyzed and studied the fungal degradation environment effect factors and degradation efficiency. The results showed that the optimum condition of fungal degradation was C:N = 5:1, pH5, temperature 30℃, strains inoculation amount 5.5ml (50ml culture medium). GC analysis was used to determine the fungal degradation effect to BTEX with the initial liquid phase concentration of 0~90mg/L, the results showed that the degradation activities of fungal strain was not inhibited, and the fungal degradation efficiency was toluene > benzene > xylene, the highest degradation efficiency reached 87.39%, 85.21%, 81.47%,respectively. The degradation efficiency of mixed substrates was slightly higher than single substrate.
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Received: 03 September 2012
Published: 25 January 2013
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[1] Cui B W,Wang L Q,Sun J J. Determinatoin of toluene and benzene in water by the wide-bore capillary column GC and the head-space sampler. Journal of Analytical Science, 2003, 19(5): 488-489. [2] 卓黎阳,舒小平,郑海涛,等. 顶空进样气相色谱法分析溶剂型木器涂料中的苯系物. 福建分析测试, 2008,17(4):40-43. Zhuo L Y, Shu X P, Zheng H T,et al.Determination of benzne, toluene and Xylene in solvent coatings for woodenware by headspace-GC.Fujian Analysis & Testing.2008,17(4):40-43. [3] Zhou J Z,Wang X L, Cao H R,et al. Benzene series in oil paint thinners determined by gas chromatography. Environmental Science & Technology, 2007, 30(1): 19-21. [4] Zhaohui Xu A M W C. Detection of Benzene,Toluene,Ethyl benzene,and Xylenes (BTEX) Using Toluene Dioxygenase-Peroxidase Coupling Reactions, 2003: 19, 1812-1815. [5] Inoue A;Horikoshi K A. Pseudomonas thrives in high concentrations of toluene.Nat,1989,338:264-266. [6] 周婷,袁世斌,邹晓莉. 一株踝节霉菌的培养特性及其在污泥生物淋滤中的应用. 应用与环境生物学报, 2012(1): 128-133. Zhou T, Yuan S B, Zou X L, Cultural characteristics of a strain of Talaromyces sp.and its application in bioleaching of sewage sludge.Chinese Journal of Applied & Environmental Biology.2012,18(1):128-133. [7] 李玮娜. 真菌生物处理"三苯"废气研究. 西安科技大学, 2007. Li W.Studies on the Treatment of Off-gases Containing BTX with Fungi.Xi'an University of Science and Technology,2007. [8] Chao H R,Wei F,Zhang L L,et al. Isolation, Identification and Biodegradation Characteristics of a Novel Chlorobenzene-degrading Bacterial Strain. Environmental Science & Technology, 2011, 34(2): 6-11. [9] Tang Y B,Wang X CH,Chen F Y,et al. Research on isolation and identification of a fluorene-degrading strain and its broad-spectrum property for degradation of polycyclic aromatic hydrocarbons. Chinese Journal of Environmental Engineering, 2011, 05(2): 467-471. [10] Crispin H.Pierce.Yill chen,Russell L Dilis,etal.Toluene metabolites as biological indicators ofexposure,Toxicology Letters,2002,129:65-76 [11] Jin Y M,Chen J M. Applications of biofiltration in air pollution control processes. Techniques and Equipment for Environmental Pollution Control, 2001, 2(3): 76-80. [12] Liao Q,Tian X,Zhu X,et al. Purifying waste gas containing low concentration toluene in trickling biofilter with ceramic spheres. Journal of Chemical Industry and Engineering(China), 2003, 54(12): 1774-1778. [13] Jin-Ying X,Hong-Ying H U,Hong-Bo Zhu,et al. Comparison of degradation properties of two strains of toluene efficient degradation bacteria. China Environmental Science, 2005, 25(z1): 102-105. [14] 尤志杰.微量液—液萃取气相色谱法测定水中的苯系物. 环境与开发, 1999(1):50. You Z J.Determination of benzene series in water with microliquid-liquid extraction cas Chromatography.Environment and Exploitation,1999(1):50. |
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