Isolation and Identification of Liver Cancer Stem Cells and Analysis of Differentially Expressed MicroRNAs

China Biotechnology

2013, Vol. 33

Issue (1): 1-7 DOI:

Isolation and Identification of Liver Cancer Stem Cells and Analysis of Differentially Expressed MicroRNAs

XU Ying-chen¹, GUAN Li-dong^1,2, ZHOU Jun-nian¹, ZENG Quan¹, YUAN Hong-feng¹, LI Si-ting¹, GUAN Zhao-xuan¹, HE Li-juan¹, NAN Xue¹, CHEN Lin¹, YUE Wen¹, PEI Xue-tao¹

1. Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China;
2. National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing 100050, China

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Abstract The aim of the study is to isolate and identify liver cancer stem cells and screen the differentially expressed microRNAs. CD90⁺ cells were confirmed the existence in eight hepatocellular carcinoma cell lines and specimens by flow cytometry and immunofluorescence. Next, CD90⁺ cells isolated from MHCC97-H cell line by MACS. Drug chemoresistance assay showed CD90⁺ cells have a stronger drug resistance capacity than CD90^- cells. CD90⁺ cells also have a stronger ability to initiate tumor formation than CD90^- cells in a xenograft model in nude mice. Taken together, liver cancer stem cells may be enriched in CD90⁺ populations. Therefore, we examined the differentially expressed microRNAs between CD90⁺ cell and CD90^- cells by means of microRNA chip. It is revealed that 92 microRNAs are differentially expressed between CD90⁺ cell and CD90- cells, of which 52 microRNAs are highly expressed in CD90⁺ cells and 40 microRNAs are highly expressed in CD90^-cells.

Key words： Hepatocellular carcinoma Cancer stem cells CD90 microRNA

Received: 08 October 2012 Published: 25 January 2013

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	Articles by authors
	XU Ying-chen
	GUAN Li-dong
	ZHOU Jun-nian
	ZENG Quan
	YUAN Hong-feng
	LI Si-ting
	GUAN Zhao-xuan
	HE Li-juan
	NAN Xue
	CHEN Lin
	YUE Wen
	PEI Xue-tao

Cite this article:

XU Ying-chen, GUAN Li-dong, ZHOU Jun-nian, ZENG Quan, YUAN Hong-feng, LI Si-ting, GUAN Zhao-xuan, HE Li-juan, NAN Xue, CHEN Lin, YUE Wen, PEI Xue-tao. Isolation and Identification of Liver Cancer Stem Cells and Analysis of Differentially Expressed MicroRNAs. China Biotechnology, 2013, 33(1): 1-7.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I1/1

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