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| Detection of Gadiformes by Real-time PCR Assay |
| LI Fu-wei1,2, ZHANG Shu-ya1, REN Shuo1, ZHANG Rui-hao1, JIANG Yu1, GU Shun-zhang1, GE Xin1, ZHANG Lei1, BAO Jian-qiang2 |
1. Shanghai Entry-Exit Inspection and Quarantine, Shanghai 200135, China;
2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China |
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Abstract A rapid and sensitive TaqMan real-time PCR assay were developed for the detection of gadiformes. For high gadiformes specificity, the primers and the probe were designed on the basis of the mitochondrial 16S rRNA gene. The detection limit of this real-time PCR assay was 0.01 ng/μl DNA concentration and 0.01%(W/W)gadiformes meat powder. To confirm the specificity of these methods, genomic DNA samples from 71other species were examined; no amplification products were detected. Thus, the method shows high sensitivity and may be applicable as specific tools for the detection of trace amounts of gadiformes in commercial food products.
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Received: 01 August 2012
Published: 25 December 2012
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Cite this article:
LI Fu-wei, ZHANG Shu-ya, REN Shuo, ZHANG Rui-hao, JIANG Yu, GU Shun-zhang, GE Xin, ZHANG Lei, BAO Jian-qiang. Detection of Gadiformes by Real-time PCR Assay. China Biotechnology, 2012, 32(12): 80-85.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I12/80
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