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| Study of Human PDGFR β Promoter in Different Human Breast Cancer Cells |
| XIE Qiu-ling, LU Jia, LIU Lan, ZHANG Chuan-yu, GUO Xin-yong, PENG Wen-dan, CHEN Xiao-jia |
| Institute of Bioengineering,Jinan University,Key Laboratory of Bioengineering Medicine of Guangdong Province,Guangzhou 510632,China |
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Abstract In order to determine the basal expression and transcription activites of PDGFRβ promoterin two kinds of breast cancer cells MCF-7 andMDA-MB-231.The basal expression of PDGFRβ in MDA-MB-231 was identified by Western blot and Real-time PCR. Five deletion mutations of human PDGFR-β promoter region were obtained by PCR from human genomic DNA and then cloned into pGL3-basic vector based on Dual-Luciferase reporter system. Two human breast cancer cells MCF-7 and MDA-MB-231 were transfected by the recombinant reporters .Electrophoretic mobility shift assays(EMSA) was used to determine the DNA binding activities of transcription factors.As a result ,the region(+539bp,+1457bp) showed a positive regulatory role,while the(+54bp,+539bp)was negative.The(－983bp ,+54bp)was found to play a significant positive role in MDA-MB-231 whereas there was no effect in MCF-7.The transcription factor AP-1 was observed to highly express in MDA-MB-231 comparing with MCF-7.AP-1 was identified to bind the PDGFRβ promoter region and its binding was higher in MDA-MB-231 than in MCF-7.The fact demonstrate that basal expression and transcription activities of PDGFRβ promoter were different in two types of breast cancer cell and AP-1 played an important role in regulatory of PDGFRβ promoter in breast cancer cells.
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Received: 11 November 2010
Published: 26 April 2011
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