Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (05): 110-115    DOI: Q789
    
Infectious Cloning Approach and Application for Varicella-Zoster Virus Based on Bacterial Artificial Chromosomes
CHUAI Xia,TAN Wen-jie
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052,China
Download: HTML   PDF(786KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Varicella-zoster virus (VZV) is a ubiquitous human alphaherpesvirus that causes varicella (chickenpox) during primary infection, can establish latency in sensory ganglia, and may reactivate to cause herpes zoster (shingles). Though VZV possesses the smallest genome among all human herpesviruses,little was known about its gene function and the attenuation mechanisms of VZV vaccine due to the difficulty of generating mutants to investigate gene function and the long period of time needed to establish pure clones using conventional techniques with mammalian cells. Recently,a completely new approach for full-length infectious clones of VZV based on bacterial artificial chromosomes(BACs) has been developed.This technique allows the maintenance,propagation and genetic modification of the viral genome as a BAC plasmid in E.coli,thus making the procedures fast,safe and effectivein prokaryotic cells.This technique also makes it possible for the reconstitution of viral progeny or mutants by transfection of the BAC plasmid into eukaryotic cells,thereby facilitating the an alysis of viral gene functions in the context of genome.The VZV BAC system also was useful as a vector for construction of recombinant live vaccines.



Key wordsVaricella-zoster virus (VZV)      Bacterial artificial chromosome(BAC)      Infectious clone     
Received: 01 February 2010      Published: 25 May 2010
Cite this article:

CHUAI Xia, TAN Wen-Jie. Infectious Cloning Approach and Application for Varicella-Zoster Virus Based on Bacterial Artificial Chromosomes. China Biotechnology, 2010, 30(05): 110-115.

URL:

https://manu60.magtech.com.cn/biotech/Q789     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I05/110

[1] Arvin A M.Varicellazoster Virus//David M K,Peter M H.Fields Virology.Philadelphia:LippincottRaven Publishers,2001:27312767. 
[2] Hosoda F, Nishimura S, Uchida H, et al. An F factor based cloning system for large DNA fragments. Nucleic Acids Res, Res. 1990,18(13):3863 3869. 
[3] Shizuya H, Birren B, Kim U J, et al. Cloning and stable maintenance of 300 kilobase pair fragments of human DNA in Escherichia coli using an F factor based vector. Proc Natl Acad Sci USA, 1992,89(18):8794 8797. 
[4] Ioannou P A, Amemiya C T, Garnes J,et al. A new bacteriophage P1 derived vector for the propagation of large human DNA fragments. Nat Genet, 1994,6(1):84 89. 
[5] Woo S S, Jiang J, Gill B S, Paterson A H, et al. Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor. Nucleic Acids Res,1994,22(23):4922 4931. 
[6] Hess N, Franz T, Kollek R, et al. Molecular cloning of Rauscher spleen focus forming virus and biological properties of the cloned virus. J Gen Virol,1984,65 (12):2225 2235. 
[7] Messerle M,Cmkovic I,Hanunerschmidt W,et al.Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromotome. Prec Nad Acad Sci USA,1997,94(26):14759 14763. 
[8] Zhou F, Li Q, Gao S J. A sequence independent in vitro transposon based strategy for efficient cloning of genomes of large DNA viruses as bacterial artificial chromosomes. Nucleic Acids Res, 2009,37(1):e2. 
[9] Creed R, Satyaprakash A, Ravanfar P. Varicella zoster vaccines. Dermatol Ther, 2009,22(2):143 149. 
[10] Nagaike K, Mori Y, Gomi Y, et al. Cloning of the varicella zoster virus genome as an infectious bacterial artificial chromosome in Escherichia coli. Vaccine,2004,22(29 30):4069 4074. 
[11] Yoshii H, Somboonthum P,Takahashi M, et al. Cloning of full length genome of varicella zoster virus vaccine strain into a bacterial artificial chromosome and reconstitution of infectious virus.Vaccine,2007,25(27):5006 5012. 
[12] Zhang Z, Rowe J, Wang W, et al. Genetic analysis of varicella zoster virus ORF0 to ORF4 by use of a novel luciferase bacterial artificial chromosome system. J Virol,2007,81(17):90249033. 
[13] Cohen J I, Seidel K E. Generation of varicella zoster virus (VZV) and viral mutants from cosmid DNAs: VZV thymidylate synthetase is not essential for replication in vitro. Proc Natl Acad Sci U S A,1993,90(15):7376 7380. 
[14] Niizuma T, Zerboni L, Sommer M H, et al. Construction of varicella zoster virus recombinants from parent Oka cosmids and demonstration that ORF65 protein is dispensable for infection of human skin and T cells in the SCID hu mouse model. J Virol, 2003,77(10):6062 6065. 
[15] Tischer B K, Kaufer B B, Sommer M, et al. A self excisable infectious bacterial artificial chromosome clone of varicella zoster virus allows analysis of the essential tegument protein encoded by ORF9. J Virol, 2007,81(23):13200 13208. 
[16] Tischer B K,von Einem J,Kaufer B, et al.Two step red mediated recombination for versatile high efficiency markerless DNA m anipulation in Escherichia coli. Biotechniques,2006,40(2):191 197. 
[17] Wussow F, Fickenscher H, Tischer B K. Red mediated transposition and final release of the mini F vector of a cloned infectious herpesvirus genome. PLoS One,2009,4(12):e8178. 
[18] Zhang Y, Buchholz F, Muyrers J P, et al. A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet,1998,20(2):123 128. 
[19] Yang X W, Model P, Heintz N. Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome. Nat Biotechnol,1997,15(9):859 865. 
[20] Kim S Y, Horrigan S K, Altenhofen J L, et al.Modification of bacterial artificial chromosome clones using Cre recombinase: introduction of selectable markers for expression in eukaryotic cells. Genome Res,1998,8(4):404 412. 
[21] Song M J, Hwang S, Wong W H, et al. Identification of viral genes essential for replication of murine gamma herpesvirus 68 using signature tagged mutagenesis. Proc Natl Acad Sci USA,2005,102(10):3805 3810. 
[22] Sadaoka T,Yoshii H,Imazawa T,et al. Deletion in open reading frame 49 of varicella zoster virus reduces virus growth in human malignant melanoma cells but not in human embryonic fibroblasts. J Virol,2007,81(22):12654 12665. 
[23] Chaudhuri V, Sommer M, Rajamani J, et al. Functions of Varicella zoster virus ORF23 capsid protein in viral replication and the pathogenesis of skin infection. J Virol, 2008,82(20):10231 10246. 
[24] Yamagishi Y, Sadaoka T, Yoshii H, et al.Varicella zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell to cell spread. J Virol,2008,82(2):795 804. 
[25] Zhang Z, Huang Y, Zhu H. A highly efficient protocol of generating and analyzing VZV ORF deletion mutants based on a newly developed luciferase VZV BAC system. J Virol Methods,2008,148(1 2):197 204. 
[26] Yoshii H, Sadaoka K, Matsuura M,et al. Varicella zoster virus ORF 58 gene is dispensable for viral replication in cell culture.Virol J,2008,30;5:54. 
[27] Lowe R S, Keller P M, Keech B J, et al. Varicella zoster virus as a live vector for the expression of foreign genes. Proc Natl Acad Sci USA,1987,84(11):3896 3900. 
[28] Shiraki K, HayakawaY, Mori H, et al. Development of immunogenic recombinant Oka varicella vaccine expressing hepatitis B virus surface antigen. J Gen Virol,1991,72(Pt 6):1393 1399. 
[29] Shiraki K, Sato H, Yoshida Y, et al. Construction of Oka varicella vaccine expressing human immunodeficiency virus env antigen. J Med Virol,2001,64(2):89 95. 
[30] Heineman T C, Connelly B L, Bourne N, et al. Immunization with recombinant varicella zoster virus expressing herpes simplex virus type 2 glycoprotein D reduces the severity of genital herpes in guinea pigs. J Virol,1995,69(12):8109 8113. 
[31] Heineman T C, Pesnicak L, Ali M A, et al. Varicella zoster virus expressing HSV 2 glycoproteins B and D induces protection against HSV 2 challenge. Vaccine,2004,22(20):2558 2565. 
[32] Somboonthum P, Yoshii H, Okamoto S, et al. Generation of a recombinant Oka varicella vaccine expressing mumps virus hemagglutinin neuraminidase protein as a polyvalent live vaccine. Vaccine,2007,17,25(52):8741 8755.

No related articles found!