THE EFFECT OF NITROGEN SOURCE ON L-THREONINE FERMENTATION

China Biotechnology

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THE EFFECT OF NITROGEN SOURCE ON L-THREONINE FERMENTATION

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Abstract TThe effects of nitrogen sources on the production of L-threonine and conversion rate of glucose were studied with L-threonine production strain TRFC. The effects of different nitrogen sources on L-threonine yield and cell mass were evaluated on shake-flask scale. The results showed that ammonium sulfate and yeast extract was the optimal inorganic and organic source, respectively. High L-threonine yield was obtained when 2.0g/L yeast extract and 10g/L Ammonium sulfate were added to fermentation medium. L-threonine production was further increased by continuous feeding of 50% ammonium sulfate during metaphase and by feeding of glucose and ammonia mixture. Under these optimal conditions, the L-threonine production of 118.9g/L was achieved, and the conversion rate of glucose was 47.6%.

Key words： fed-batch fermentation L-threonine nitrogen source

Received: 23 June 2006 Published: 25 November 2006

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. THE EFFECT OF NITROGEN SOURCE ON L-THREONINE FERMENTATION. China Biotechnology, 2006, 26(11): 54-58.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I11/54

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