Cloning, Expression and enzyme characterization analysis of Aspergillus sulphureus xylanase gene xynB

China Biotechnology

2006, Vol. 26

Issue (0): 95-100 DOI:

Cloning, Expression and enzyme characterization analysis of Aspergillus sulphureus xylanase gene xynB

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Abstract

Xylanase gene xynB of Aspergillus sulphureus was amplified by RT-PCR. Sequence analysis showed that xynB composed of 678 nucleotides (nt), which encoded a 24 kDa protein, shared more than 95% similarity to the xynB sequences of A.niger opened in GenBank. xynB was overexpressed in Escherichia coli BL21 induced by IPTG after it was ligated into vector pET28a(+). The recombinant enzyme was optimally active at 40℃ and pH4.4. The xylanase activity just decreased 13% after 6 h in pH2.4 buffer. It has no obvious influence on enzyme activity after incubation at 30~50℃ for 30 min. XynB enzyme retained higher residual activity when different concentrations of Cu2+, Zn2+ and Ca2+ was added to the catalytic system respectively.

Key words： characterization analysis prokaryotic expression Aspergillus sulphureus xylanase

Received: 09 January 2006 Published: 15 June 2006

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Cite this article:

. Cloning, Expression and enzyme characterization analysis of Aspergillus sulphureus xylanase gene xynB. China Biotechnology, 2006, 26(0): 95-100.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I0/95

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