技术与方法 |
|
|
|
|
Purification of Kallikrein Using Reverse Micellar Extraction |
|
|
|
|
Abstract The purification of Kallikrein using CTAB/hexanol/octane reverse micelles extraction has been studied and optimized,under various aqueous pH values, ionic strength and species, CTAB concentration and co-surfactant concentration. The result shows that the activity recovery is more than 80%, the commercial enzyme activity is increased by 1.97 times and the crude enzyme activity is increased by 7.15 times under the conditions of[CTAB]=0.02 mol•L-1, hexanol/octane (V/V)=1:5, pH=9.0 and[KBr]=0.1 mol•L-1 in forward extraction, pH=7.0 ,[KBr]=1.5 mol•L-1 and 15% ethanol(V/V) in backward extraction. The result of purified Kallikrein is examined by the SDS-PAGE analysis. Reverse micelles extraction is a potential technique for the application in the downstream biotechnological processes.
|
Received: 23 October 2006
Published: 25 March 2007
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|