研究报告 |
|
|
|
|
The Research on Expression and Purification of a Chimeric anti-p185 antibody |
|
|
|
|
Abstract TThe Her-2 proto-oncogene encodes a 185kD transmenbrane glycoprotein p185 which has intrinsic tyrosine kinase activity. It is overexpressed in several malignant human tumors like breast cancer. We have constructed a chimeric antibody by assembling a single-chain Fv antibody and a human IgG1 Fc fragment. This chimeric antibody reacts with tumor surface antigen p185c-erbB-2 specifically. In order to put the antibody into clinical application, we use two steps purification method to attain the antibody's purity more than 95%. Then we found both the lyophilized pharmaceutical formulations of the antibody. The formulations can keep the stability and activity of the antibody for at least 1 year. These results were the foundation of the chimeric antibody for cancer therapy.
|
Received: 27 April 2006
Published: 25 September 2006
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|