Abstract Objective This experiment research on the influence of protein expressed by hTERT gene on the NSCs biological characteristics. Method In this experiment, constructed recombinant plasmid pEGFP-N1-hTERT was transfected into rat fetal NSCs with lipid-mediated transfection method Transfected NSCs differentiation in vitro tests, tumorigenicity in nude mice test, RT-PCR and Western-blot analysis and flow cytometry. Result Transfected NSCs stem cells still have the potential growth characteristics and multipotency in vitro, no tumorigenic, and transcription product of hTERT gene mRNA and hTERT-GFP fusion protein expression in the transfected NSCs. Conclusions The transferred NSCs of telomerase activity was up-regulation and the proliferation of cells were promoted in vitro by hTERT gene, and also displayed immortalization tendency.
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