Mass Culture and Purification of Vero-based Influenza Virus

China Biotechnology

2008, Vol. 28

Issue (11): 20-24 DOI:

Mass Culture and Purification of Vero-based Influenza Virus

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Abstract

Objective Establish mass-scale purification technology of cell-derived influenza virus. Methods A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion This research provides a high effective purification technology for cell-derived influenza virus.

Key words： Vero cells influenza virus purification

Received: 18 June 2008 Published: 20 April 2009

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	HE Chun-Yan
	CHEN Ze
	WU Shu-Jun
	TANG Li-Li
	QIN Ji

Cite this article:

. Mass Culture and Purification of Vero-based Influenza Virus. China Biotechnology, 2008, 28(11): 20-24.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I11/20

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