Characterization of HA Gene and NA Gene of A pigeon H5N1 Influenza A Virus

China Biotechnology

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Characterization of HA Gene and NA Gene of A pigeon H5N1 Influenza A Virus

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Abstract In this study, RT-PCR were employed to amplify the cDNAs of HA gene and NA gene of A pigeon avian influenza isolate with two pairs of primers, the cDNAs were cloned to pMD18-T and then sequenced. The results of sequence analysis shows that the cloned HA gene consists of 1707 nucleotides and encodes 568 amino acids residues and contains whole open reading frame. There are 7 Potential glycosylation sites and 6 basic amino acids（R-R-R-K-K-R） insert at the cleavage site in HA of the influenza virus isolate which is a symbol of highly pathogenic avian influenza , amino acids of receptor-binding site are YWIHELY, amino acids of left edge of receptor-binding site are SGVSSA, amino acids of right edge of receptor-binding site are NGQSGR; The cloned NA gene consists of 1350 nucleotides and encodes 446 amino acids residues,There are 3 Potential glycosylation sites in NA of the influenza virus isolate.

Key words： avian influenza virus hemagglutinin gene Neuraminidase gene sequence analysis pigeon

Received: 20 September 2005 Published: 25 May 2006

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Cite this article:

. Characterization of HA Gene and NA Gene of A pigeon H5N1 Influenza A Virus. China Biotechnology, 2006, 26(05): 17-21.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I05/17

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