中国生物工程杂志

Aloe has been deeply researched recently because of its attracting function on medical treatment, hairdressing, health protection and edibility. It was investigated that DREB transcription factor were able to improve crop the tolerance to abiotic and biotic stresses. In this experiment, aloe stems were used as explants for OsDREB gene transformation mediated by Agrobacterium. PCR analyses demonstrated that OsDREB gene was successfully transferred into aloe genome with a transformation efficiency of 0.5%. Relationship between relative electrical conductivity and cold resistance was studied through positive and negative plants treated with the same low-temperature. The results of relative electrical conductivity in positive plants were higher than the negative plants. And the leaves of negative plants appear severe evidence of freeze injury with brown, withered and translucent, as the positive plants appear good growing condition.