Optimization of Cultural Condition of Ganoderma lucidum in the Compound Culture Medium by Response Surface Methodology

China Biotechnology

2009, Vol. 29

Issue (12): 37-42 DOI:

Optimization of Cultural Condition of Ganoderma lucidum in the Compound Culture Medium by Response Surface Methodology

YU You-gui^1,2,LI Zhong-hai¹,HUANG Guo-hua²,CHEN Cai-qiong²

1.Central South University of Forestry Technology，Changsha 410004
2.Shaoyang University,Shaoyang 422000，China

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Abstract

The purpose is to obtain optimal cultural conditions for Ganoderma lucidum in the solid medium mainly containing the residual of Pteridium aquilinum,and to provide theoretical foundation for the further use of this material from Pteridium aquilinum.Response surface methodology was applied to optimize the main culture conditions including proportion of the residual compound in medium,water content in medium and culture temperature.The results showed that proportion of residual of Pteridium aquilinum in medium,water content in medium and cultural condition had noticeably significant effects on daily average growth rate of mycelium of Ganoderma lucidum(p<0.01).Obviously correlation was found to be existed between proportion of residual of Pteridium aquilinum and water content in medium,or water content in medium and the cultural temperature.The optimal proportion of residual of Pteridium aquilinum in medium was 85.0%.The optimal water content in medium was 62.4%,and the optimal cultural temperature was 27℃.Under these optimized conditions,daily average growth rate of mycelium of Ganoderma lucidum was 3.48mm/d.Multivariate regression analysis showed that the model for regressing proportion of residual of Pteridium aquilinum in medium,water content in medium,cultural temperature and daily average growth rate of mycelium of Ganoderma lucidum might be available for the culture of the fungi.Practical growth rate of mycelium of Ganoderma lucidum could be forecasted by applying the model. The application of residual of Pteridium aquilinum as a main additive in the culture of Ganoderma lucidum was firstly reported.It has laid a pave for the further investigation of residual of Pteridium aquilinum in medium.

Key words： Residual of Pteridium aquilinum Ganoderma lucidum Cultural condition Growth rate Response surface methodology

Received: 07 July 2009 Published: 21 December 2009

ZTFLH:

Q93-335

Corresponding Authors: you guiyu E-mail: yufly225@yahoo.com.cn

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	TU Wei-Gui
	LI Zhong-Hai
	HUANG Guo-Hua
	CHEN Cai-Qiong

Cite this article:

TU Wei-Gui, LI Zhong-Hai, HUANG Guo-Hua, CHEN Cai-Qiong. Optimization of Cultural Condition of Ganoderma lucidum in the Compound Culture Medium by Response Surface Methodology. China Biotechnology, 2009, 29(12): 37-42.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I12/37


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