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| The Experimental Study of Repairing Laryngic Catilage Defect by Transplanting the Compound of Bone Mesenchymal Stem Cells and PLGA Porous Foam Scaffolds |
| MA Lin-xiang1,CUI Ying2,ZHU Li-ming1,PAN Xin-yu1,WANG Xue-feng2,ZHANG Ben2 |
1.Department of Otolaryngology in Liaoning Medical College,Jinzhou 121001,China
2.Department of Otolaryngology,The First Affiliated Hospital of Liaoning Medical College,Jinzhou 121001,China |
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Abstract Objective:To evaluate the feasibility of repairing laryngic catilage deffect by mesenchymal stem cells and PLGA porous foam scaffolds under the effect of cartilage-derived morphogenetic protein 1 and transforming growth factor-β1 by tissue engineering techniques. Methods:In vitro ,under the effect of CDMP1 and TGF-β1 ,BMSCs and PLGA porous foam scaffolds were cultured to investigace the expression of cartilaginous phenotype The histological staining for glycosaminoglycan using alcian blue dye-binding method and immunohistochemical staining of cartilage-specific protein collagen Ⅱ were used to identify chondrogenic differentiation of BMSCs. Results:BMSCs cultured in the medium containing CDMP1 and TGF-β1 expressed collagen Ⅱ and glycolsaminoglycan. Transplanted into the body ,BMSCs - Biology stent can repair laryngic cartilage defects. Conclusion:under the dffect of CDMP1 and TGF-β1,BMSCs-Biology stent can differentiate into the chondrogenic phenotype and can effectively repair laryngic cartilage defects.
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Received: 06 July 2009
Published: 21 December 2009
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