N-terminal of α-Synuclein Involved in Regulation of Mitochondrial Function

China Biotechnology

2009, Vol. 29

Issue (12): 1-6 DOI:

N-terminal of α-Synuclein Involved in Regulation of Mitochondrial Function

LV Wang-Le,ZHANG Tao,LIU Qi,FAN Chun-xiang,ZHANG Ling,ZHAO Huan-ying,ZHAO Chun-li,YANG Hui

Beijing Institute for Neuroscience, Capital Medical University, Beijing Center of Neural Regeneration and Repair, Key Laboratory of Neurodegenerative Diseases, Ministry of Education， Beijing 100069, China

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Abstract

Objective: To identify the functional domain of α-Synuclein in affecting mitochondrial function and how the function to be impaired, especially, the mitochondrial membrane potential and the release of Cytochrome c. Methods: Harvest of α-Syn-N and α-Syn-△N by PCR, then subcloned into the pCMV-Myc mammalian expression vector. The recombinant plasmids were transfected into HEK293T cells by Lipofectamine 2000. After detecting the protein expression by western blot, the functional domain was detected by co-immunoprecipitation. The mitochondrial membrane potential through flow cytometry and immunofluorescence, at the same time, the release of Cytochrome c through flow cytometry to detect. Results: The recombinant plasmids were constructed successfully. CO-IP has proved that N-terminal may be the functional domain of α-Synuclein in affecting mitochondria. Over-expression of N-terminal could depolarize the mitochondrial membrane potential and induce the Cytochrome c releasing in MN9D cells. Conclusion: N-terminal may be the functional domain of a-synuclein and over-expression of N-terminal could decrease mitochondrial activity.

Key words： N-terminal of α-synuclein mitochondria mitochondrial membrane potential Cytochrome c

Received: 01 June 2009 Published: 21 December 2009

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R742.5

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	Articles by authors
	LV Wang-Le
	ZHANG Tao
	LIU Qi
	FAN Chun-Xiang
	ZHANG Ling
	DIAO Huan-Yang
	DIAO Chun-Li
	YANG Hui

Cite this article:

LV Wang-Le, ZHANG Tao, LIU Qi, FAN Chun-Xiang, ZHANG Ling, DIAO Huan-Yang, DIAO Chun-Li, YANG Hui. N-terminal of α-Synuclein Involved in Regulation of Mitochondrial Function. China Biotechnology, 2009, 29(12): 1-6.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I12/1


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