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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (02): 120-126    DOI: Q78
    
The Advances of Transgenic Plants Detection Techniques
College of Life Science, Northwest University, Xi’an 710069,China
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Abstract  

With the development of genetic engineering, more and more transgenic plants and their products released to people's lives and greatly changed our lives. The controversy over potential ecological risks and the impact of transgenic plants on human health are becoming increasingly acute. Therefore, the techniques for detecting transgenic plants must be established and perfected.The advances in detection techniques of transgenic plants were summarized. The progresses of PCR, ELISA as well as gene chip in transgenic plants detection were emphasized,and their characteristics were compared. Furthermore, detection on specific metabolites in transgenic plants was also introduced. Based on detection of the differential protein, one project of two-dimensional electrophoresis technology for traceability of genetically modified organisms and products was put forward finally.



Key wordsTransgenic plants      Detection techniques      Gene chipPCR      Two-dimensional electrophoresis     
Received: 21 October 2009      Published: 26 February 2010
Cite this article:

GUO Bin, QI Xiang, WEI E-Hui. The Advances of Transgenic Plants Detection Techniques. China Biotechnology, 2010, 30(02): 120-126.

URL:

https://manu60.magtech.com.cn/biotech/Q78     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I02/120

[1] Organization for Economic Cooperation and Development OECD Safety evaluation of foods derived by modem biotechnology: Concepts and Principles. Pairs,OECD,1993. 
[2] Wolf C, Scherzinger M, Wurz A, et al Detection of cauliflower mosaic virus by the polymerase chain reaction: testing of food components for falsepositive 35Spromoter screening results.Eur Food Res Technol, 2000, 210: 367372. 
[3] 黄昆仑, 罗云波. 用巢式和半巢式PCR检测转基因大豆Roundup Ready及其深加工食品. 农业生物技术学报, 2003, 11(5): 461466. Huang K L, Luo Y B. J Agricul Biotech, 2003, 11(5): 46l466. 
[4] CarciaCanas V, Gonzalez R, Cifuentes A. Sensitive and simultaneous analysis of five transgenic maizes using multiplex polymerase chain reaction, capillary gel electrophoresis, and laserinduced fluorescence Electrophoresis, 2004, 25(14): 22192221. 
[5] 张茎, 王淑芳, 赵彦修, 等. HAL1基因转化番茄及耐盐转基因番茄的鉴定. 生物工程学报, 2001, 17(6):658662. Zhang J, Wang S F, Zhao Y X, et al Chin J Biotech, 2001, 17(6): 658662. 
[6] 张洪瑞, 朱其松, 宋克勤, 等. 转基因食品的安全性评价与检测技术. 河北农业科学, 2008, 12(9): 101103, 105. Zhang H R, Zhu Q S, Song K Q, et al.J Hebei Agricul Sci, 2008, 12(9): 101103, 105. 
[7] 陈颖, 徐宝梁, 苏宁, 等. 实时荧光定量PCR技术在转基因玉米检测中的应用研究. 作物学报. 2004, 30 (6): 602607. Chen Y, Xu B L, Su N, et al.AAS, 2004, 30(6): 602607. 
[8] Thomas D, Nardone G, Randall S. Amplification of padlock probes for DNA diagnostics by cascade rolling circle amplification or the polymerase chain reaction.Arch Pathol Lab Med, 1999, 123: 11701176. 
[9] 陶震, 蔡兴锋, 颜志强, 等. HRCA技术在转基因植物检测中的应用. 生物工程学报, 2003, 19(3): 294300. Tao Z, Cai X F, Yan Z Q, et al Chin J Biotech, 2003, 19(3): 294300. 
[10] Brett G M, Chambers S J, Huang L, et al.Design and development of immunoassays for detection of proteins Food Contro1, 1999, 10: 401406. 
[11] Notomi T, Okayama H, Masubuchi H, et al.Loopmediated isothermal amplification of DNA Nucleic Acids Res, 2000, 28(12): 63. 
[12] Shivappa R B, Savan R, Kono T, et al.Detection of spring viraemia of carp virus (SVCV) by loopmediated isothermal amplification (LAMP) in koi carp, Cyprinus carpio L. J Fish Dis, 2008, 3l(4): 249258. 
[13] 赵飞, 邹为民. LAMP法在水产动物病原快速检测中的应用. 南方水产, 2007, 3(2): 7175. Zhao F, Zou W M. South China Fish Sci, 2007, 3(2): 7175. 
[14] 兰青阔, 王永, 赵新, 等. LAMP在检测转基因抗草甘膦大豆cp4epsps基因上的应用. 安徽农业科学, 2008, 36(24): 1037710378, 10390. Lan Q K, Wang Y, Zhao X, et al. Journal of Anhui Agri Sci,2008, 36(24): 1037710378, 10390. 
[15] Lipton C R. Guidelines for the validation and use of immunoassays for determining of introduced proteins in biotechnology enhanced crops and derived food ingredients. Food Agric Immunol, 2000, 12: 153164. 
[16] 王莉江, 明小天, 安成才, 等. 籼稻明恢63成熟种子愈伤组织的诱导及转基因水稻的抗性检测. 生物工程学报, 2002, 18(3): 323327. Wang L J, Ming X T, An C C, et al. Chin J Biotech, 2002, 18(3): 323327. 
[17] 阚贵珍, 喻德跃. 试纸条法和PCR法检测抗草甘膦转基因大豆的外源基因. 中国油料作物学报, 2005, 27(4): 1821. Kan G Z, Yu D Y. Chin J Oil Crop Sci, 2005, 27(4): 1821. 
[18] Weinberger S R, Dalmasso E A, Fung E T. Current achievements using protein chip array technology.Curr Opin Chen Biol, 2002, 6(1): 8691. 
[19] 贾洪革, 吕玲飞, 庞永奇, 等. 用绿色荧光蛋白监测转基因植物中选择标记基因的消除. 生物工程学报, 2004, 20(1): 1015. Jia H G, Lü L F, Pang Y Q, et al. Chin J Biotech, 2004, 20(1): 1015. 
[20] Germini A, Rossi S, Zanetti A, et al. Development of a peptide nucleic acid array platform for the detection of genetically modified organisms in food. J Agric Food Chem, 2005, 53(10): 39583962. 
[21] Bordoni R, Mezzelani A, Consolandi C, et al. Detection and quantitation of genetically modified maize (Bt176 transgenic maize) by applying ligation detection reaction and universal array technology J Agric Food Chem, 2004, 52(5): 10491054 . 
[22] 尤元海, 张建中. 基因表达谱芯片的数据挖掘. 中国生物工程杂志, 2009, 29(10): 8791. You Y H, Zhang J Z. China Biotech, 2009, 29(10): 8791. 
[23] 李荣. 基因表达谱芯片数据挖掘系统. 计算机应用研究, 2009, 26(8): 293829411. Li R. App Res Comput, 2009, 26(8): 293829411. 
[24] Byrdwell W C, Neff W E. Analysis of genetically modified canola varieties by atmospheric pressure chemical ionization mass spectrometric and flame ionization detection. J Liq Chromatogr Related Technol, 1996, 19: 22032225. 
[25] Hurburgh C R. Measurement of soybean fatty acid content with nearinfrared spectroscopy. Annual Meeting.Kansas City, MO, American Oil Chemists Society,2003, 1: 59. 
[26] 陈永红, 李哲敏, 许世卫. 农业转基因生物安全性分析. 中国食物与营养, 2003, 9: 45. Chen Y H, Li Z M, Xu S W. Food Nut China, 2003, 9: 45 . 
[27] 姜先荣, 杨杰, 周衍茂. 转基因技术应用及转基因产品安全性研究进展. 合肥师范学院学报, 2008, 26(3): 99103. Jiang X R, Yang J, Zhou Y M. J Hefei Teach College, 2008, 26(3): 99103. 
[28] Konig A. A framework for designing transgenic crops: science, safety and citizen's concerns. Nat Biotechnol, 2003, 21: 12741279. 
[29] Stewart C N, Halflaill M D, Warwick S I. Transgene introgression from genetically modified crops to their wild relatives Nat Rev Genet, 2003, 4(10): 806817. 
[30] 王旭静, 贾士荣. 国内外转基因作物产业化的比较. 生物工程学报,2008, 24(4): 541546. Wang X J, Jia S R. Chin J Biotech, 2008, 24(4): 541546. 
[31] Virginia G C, Ramon G, Alejandro C. The combined use of molecular techniques and capillary electrophoresis in food analysis. Trends Anal Chem, 2004, 23(9): 637643. 
[32] Hernandez M, RodriguezLazaro D, Esteve T, et al. Development of melting temperaturebased SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection. Anal Chem, 2003, 323(2): 164170. 
[33] Sutherland I A and Fisher D. Role of countercurrent chromatography in the modernization of Chinese herbal medicines. J Chromatography A, 2009, 1216: 740753. 
[34] Feriotto G, Borgatti M, Mischiati C, et al. Biosensor technology and surface plasmon resonance for realtime detection of genetically modified Roundup Ready soybean gene sequences. J Agric Food Chem, 2002, 50: 955962. 
[35] Roussel S A, Hardy C L, Hurburgh C R, et al.Detection of roundup ready soybean by nearinfrared spectroscopy. Appl Spectrosc, 2001, 55(10): 1425l430. 
[36] 张国安, 许雪姣, 张素艳, 等. 蛋白质组的分离与分析及其应用进展. 分析化学, 2003, 31(5): 345354. Zhang G A, Xu X J, Zhang S Y, et al. Chin J Analyt Chem, 2003, 31(5): 345354.

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