|
|
|
| The Fluorescent Marker Development Based on MegaBACE 1000 DNA Sequencing System |
| LIU Xian-de1,2,ZHANG Guo-fan1 |
1.Chinese Academy of Sciences, Institute of Oceanology, Qingdao 266071, China
2.Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University,Fisheries College, Jimei University, Xiamen 361021, China |
|
|
|
Abstract The MegaBACETM 1000 DNA Sequencing System was made by Amersham Pharmacia, which is a high throughput fluorescence-based DNA sequencer that can be used for DNA sequencing and genetic analysis etc. But the markers（ET550-R Size Standards）produced by this corporation which are used for genetic analysis in this instrument are very expensive. In order to cut the cost,a set of markers were developed and after authentication,that can be used in the MegaBACE 1000 DNA sequencer. Using these markers and our own recognition software,an improved, high throughput AFLP work protocol based on MegaBACETM 1000 were constructed.
|
|
Received: 07 May 2009
Published: 21 December 2009
|
|
|
|
|
| ［1］ |
Liu X D, Liu X, Guo X, et al. A preliminary genetic linkage map of the pacific abalone Haliotis discus hannai Ino. Marine Biotechnology, 2006, 8: 386~397
|
|
|
| ［2］ |
GeneScan Reference Guide, Chemistry Reference for the ABI Prism 310 genetic Analyzer. PE applied system. Chapter 5, 1~17
|
|
|
| ［3］ |
MegaBACE 1000 Instrument Administrator’s Guide Version 2.0, Amersham Biosciences, Appendix A Fluorescence imaging, pA1~11
|
|
|
| ［4］ |
Igor L Medintz, Lorenzo Berti, Charles A Emrich, et al. Genotyping energytransfercassettelabeled ShortTandemRepeat Amplicons with Capillary Array Electrophoresis Microchannel Plates, Clinical Chemistry,2001, 47(9):1614~1621
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
