中国生物工程杂志

Objective: To construct the eukaryotic expression vector for DINH and sC3d3 fusion gene and express it in vitro．Methods: The fragment of C3dwas amplified by RT-PCR and inserted into pMDT-18 plasmid and then sub cloned into eukaryotic expression vector pcDNA-DPPIS-DINH-mC3d3.The recombinant plasmid pcDNA-DPPIS-DINH-sC3d3 was transfected into BHK-21 cells and the expressed product was detected by Western blot. Results: Western blot demonstrated that DINH and sC3d3 existed in the cultural supernatant．Conclusion: The constructed DNA vaccine can be expressed in vitro, which may pave a way for further studies in animals.