中国生物工程杂志

Site-saturation mutagenesis is a newly-developed technology in protein engineering. By manipulating the encoding genes, it can rapidly obtain the mutants of desired proteins whose target residues are substituted by 19 other common amino acids. Site-saturation mutagenesis could serve not only as a powerful tool in protein engineering, but also as an important method in exploring the structure-function relationship of proteins. In this review, we summarize several techniques to achieve site-saturation mutagenesis and introduce their application status in the protein engineering. The problem and promising future of its application were also discussed.