中国生物工程杂志

Objective To establish a quick and simple method used for evaluating capability of sperm cells up-take exogenous DNA. Methods After co-cultured with digoxigenin (DIG) labeled DNA, frequencies of sperm cells carrying foreign DNA were tested by immunocytochemistry and compared with corresponding results from liquid scintillation counting. Results Sperm cells carrying exogenous DNA were dyed heavily red and control sperm cells did not. At the same time, the process and location of exogenous DNA interacted with sperm cells were also displayed. Furthermore, results came from immunocytochemistry had obviously relationship with that that from liquid scintillation counting, a classical method to evaluate sperm capability to take up foreign DNA (r = 0.96). Conclusions Imunocytochemistry is not only a safe, quickly and sensitive method in estimating the capability of sperm cells to take up exogenous DNA, but more information about interaction between sperm cells and exogenous DNA could also be revealed.