Cloning and Sequencing of Suaeda hetroptera Kitag CMO cDNAand Construction of its recombinant Plant Expression Vector

China Biotechnology

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Cloning and Sequencing of Suaeda hetroptera Kitag CMO cDNAand Construction of its recombinant Plant Expression Vector

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Abstract Total RNA was extracted from leaf of Suaeda hetroptera Kitag, then the CMO(choline monooxygenase)cDNA was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) method and cloned into pMD-T-simple vector. The positive clones from the Blue/White Screen were sequenced. After confirming its validity, we subcloned the CMO gene fragment into pBI121 vector. Double enzymes restriction and PCR analysis indicated that the pBI121/CMO recombinant plasmid was successfully constructed.

Key words： choline monooxygenase pMD18-T-simple Vector pBI121 Plant Expression Vection E.coli JM109

Received: 29 November 2005 Published: 25 July 2006

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Cite this article:

. Cloning and Sequencing of Suaeda hetroptera Kitag CMO cDNAand Construction of its recombinant Plant Expression Vector. China Biotechnology, 2006, 26(07): 80-83.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I07/80

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