Cloning and Expression of Vivrio Cholerae CTB Gene and the Recombinant CTB Protein Activation Assay

China Biotechnology

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Cloning and Expression of Vivrio Cholerae CTB Gene and the Recombinant CTB Protein Activation Assay

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Abstract CTB protein possessed mucosal adjuvant immunoactivity. The CTB gene was amplified by PCR method from a strain V. cholerae. The nucleotide sequence of CTB gene was 375 bp and shared 96.0%~99.2% homology with other 6 CTB genes. The recombinant plasmid pTWIN1-CTB was transformed E. coli strain BL21(DE3) and was expressed with 0.8 mmol/L IPTG. The molecular weight of expression products was identical with expectative weight by SDS-PAGE electrophoresis. The CTB fusion proteins mainly assembled inclusion bodies and the outputs of proteins were approximately 20% of the total bacterial proteins. The CTB proteins possessed mucosal immunoactivity by GM1-ELISA assay.

Key words： expression of CTB gene GM1-ELISA Vibrio Cholerae CTB gene

Received: 29 May 2006 Published: 25 October 2006

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Cite this article:

. Cloning and Expression of Vivrio Cholerae CTB Gene and the Recombinant CTB Protein Activation Assay. China Biotechnology, 2006, 26(10): 13-17.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I10/13

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