DNA shuffling and molecular evolution

China Biotechnology

2006, Vol. 26

Issue (0): 153-156 DOI:

DNA shuffling and molecular evolution

Download:

HTML

PDF(395KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

DNA shuffling is a practical molecular breeding process for the rapid evolution of genes by in vitro recombination. It has extended its researches and applications to a wide areas, including the improvement of the structure and function of proteins, specificity and activity of enzymes, small molecular biochemical pharmaceuticals and drug metabolic enzymes, gene therapy vehicles and transgenes, laboratory animal models and so on. In addition, DNA shuffling is also used as an effective theoretical researching tool in molecular evolution. Here we gave reviews in molecular evolution, especially the technology of DNA shuffling and its main applications and development.

Key words： DNA shuffling in vitro recombination molecular evolution EP-PCR

Received: 13 March 2006 Published: 15 June 2006

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. DNA shuffling and molecular evolution. China Biotechnology, 2006, 26(0): 153-156.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I0/153

[1]	HE Lin-wei, LIU Zhang-min, FENG Yan, CUI Li. High Throughput Screening Method and Application for L-glutamate Specific Aminotransferase[J]. China Biotechnology, 2017, 37(8): 59-65.

[2]	WU Xiu-xiu, LV Xiao-hui, HU Ya-dong, XIE Chun-fang, LIU Da-ling, YAO Dong-sheng. *Directed Evolution in vitro* of Armillariella tabescens MAN47 β-Mannanase with Higher Thermalstability and Acid Tolerance**[J]. China Biotechnology, 2012, 32(03): 83-90.

[3]	TIAN Ya-rong, WANG Ying-hui, XU Mei-ai, LIN Jun, LIN Juan, YE Xiu-yun. Construction and Evaluation of a Directed Evolution Library of Catalase[J]. China Biotechnology, 2011, 31(9): 82-87.

[4]	HEI Qian, XU Yuan, GUO Li-rong, HUANG Ji-bing, ZHANG Hui, XIA Tao. *Overexpression of A New Evolved Na⁺/H⁺ Antiporter Gene AtNHXS1* Improved Salt Tolerance of Tobacco(Nicotiana tabacum L.)**[J]. China Biotechnology, 2011, 31(8): 24-28.

[5]	CHAN Shi-Beng, JIA Li-Qiu, DING Hua-Zhi, LIU Dan-Quan, ZHANG You-Meng. Research on the Latest Developments and Application of DNA Shuffling[J]. China Biotechnology, 2009, 29(10): 92-97.

[6]	Chun Li . Development of Studies and Application in Fermentation of Vitreoscilla Hemoglobin[J]. China Biotechnology, 2008, 28(7): 133-138.

[7]	. The Progress and Application of Recombinant PCR[J]. China Biotechnology, 2007, 27(5): 153-156.

[8]	. Molecular Cloning and Sequence Analyzing of COⅢ Gene in Duck[J]. China Biotechnology, 2007, 27(3): 54-49.

[9]	. DNA shuffling of Vitreoscilla Hemoglobin[J]. China Biotechnology, 2006, 26(11): 14-19.

[10]	. DNA shuffling of Arabidopsis thalianna K+ uptake transporter gene[J]. China Biotechnology, 2006, 26(07): 25-30.

Viewed

Full text

Abstract

Cited

Shared

Discussed