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Application of Colloidal Gold Immunochromatography Assay, DIA and PCR for detection of CBCD |
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Abstract The sensitivity, specificity and application and expenses among the gold immunochromatography assay (CGIA), DIA (Dot Immunobinding Assay) and PCR assay have been compared. Use trisodium citric acid deoxidization to prepare colloidal gold, and then it was coupled with rabbit polyclonal antibody of Xac04 pathogen, to produce the fast reagent strip of CGIA. A new useful method for rapid screening assay of CBCD in field was developed.
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Received: 24 February 2006
Published: 15 June 2006
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