中国生物工程杂志

As a real-time, fast and label-free instrument for biomolecular interactions, surface plasmon resonance biosensor has been widely used in biomedical analysis and research. Carboxymethylated dextran modified CM5 sensor chip is the core of the Biacore instrument. CM5 sensor chips are now mostly purchased from Pharmacia Biosensor AB (Uppsala Sweden), which are expensive and once the covalent immobilized receptor molecules have lost bioactivity, it cannot be reused.An easy, cost-effective method for regeneration, characterization and preparation of carboxymethylated dextran modified (CMD) sensor chips for SPR biosensor was described. The prepared CMD surface was well characterized by cyclic voltammetry and atomic force microscopy and successfully used for the detection of prostate-specific antigen in solution and the measurement of the constants of affinity and kinetics of the binding of PSA with PSA antibody.