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Embryonic Stem Cells Differentiated To Neural Cells By High-concentration RA Induced |
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Abstract Objective: To investigate the effect of high-concentration RA (all-trans retinoic acid,RA) on the differentiation of ESC. Methods: During the formation of embryoid bodys, exposured to 5μM RA. Neural precursor cells were replated in different substrates. Immunofluorescence analysed the different stage of ESC differentiation. Results: 5μM RA treatment of differentiating ESC was important for the induction of nestin positive cells. Approximately 76% of the cells were β-tubulin III positive, 55% of β-tubulin III positve cells were GABA positive when cultured on LPO, whereas only 4% were CHAT positive. Conclusion: High-concentration RA strongly drived neural induction.
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Received: 10 December 2006
Published: 25 May 2007
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