中国生物工程杂志

Using RT-PCR technique, the entire FMDV-3C gene segment was amplified from suckling mouse carcass and cloned into pGEM T easy vehicle to obtain the recombinant plasmid pT-3C, which was confirmed by nucleotide sequencing. EcoRI cleavage was used to construct a 3C protease fusion expression vehicle, and recombinants having the correct orientation were screened with a pair of primers Pr78/3C-A2. The prokaryotic expression vehicle pSOC-3 C constructed was subjected to restriction analysis and, under the induction of IPTG, could in E.coli BL21 (DES)plysS express the SOC-3C fusion protein that gave a 26 KD-sized target electrophoretic band, the latter exhibiting immunoreactivity on Western-blotting. These findings paved the way for further research in bacteriophage surface display of the 3C protease and development of novel vaccines and pharmaceuticals