TILLING IN RICE BREEDING IN FUTURE

China Biotechnology

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TILLING IN RICE BREEDING IN FUTURE

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Abstract Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics method for functional genomics research.It is possible to screen for point mutations in the populations of EMS mutagenesis with high-throughput and low-cost. EcoTILLING is a method based on TILLING ,was developed for detecting multiple types of polymorphisms in germplasm collections,such as single nucleotide polymorphism,small deletion and insertion etc.Rice is a very important food crop.It is a model plant for genome research also,there are complete genome sequence and a lot of other bioinformatics resources about it.So the marker-assisted breeding is becomeing more and more important in rice breeding.In this paper ,we will discuss some issues based on TILLING about identify germplasm based on gene sequence,EMS mutagenesis breeding,developing functional marker in rice breeding in future.

Key words： rice germplasm Identification EMS mutagenesis TILLING

Received: 13 March 2006 Published: 25 November 2006

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. TILLING IN RICE BREEDING IN FUTURE. China Biotechnology, 2006, 26(11): 76-80.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I11/76

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