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| The Establishment of Quantitative ELISA of Foot-and-Mouth Disease Virus Non-structural Protein 3B |
| LI Yong-liang1, LU Zeng-jun1,YANG Su-zhen2,TIAN Mei-na1,XIE Bo-xia1,LIU Zai-xin1 |
1. Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Sciences, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory,Lanzhou 730046, China
2.Henan Key Lab for Animal Immunology of Henan Academy of Agriculture Sciences, Zhengzhou 450002, China |
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Abstract Objective:Establish an ELISA method for quantitative detection of non-structural protein 3B in the FMD virus culture medium using monoclonal antibody.Methods:At first, monoclonal antibody was binding with the test virus culture medium overnight, and then transferred to the ELISA plate coated with 3B protein, using standard protein 3B in control, while establishment negative and blank control. The content of non-structural protein 3B in the culture medium was calculated by regression analysis finally.Results:The regression curve was typical of s-type and fitted with the 4 parameter logic curves. The correlation coefficient R was 0.99 and the range of detection was 5~1500ng/ml while the half inhibitory concentration (Ic50) was 130ng/ml.Conclusion:This method is specific, sensitive and quantitative to detect non-structural protein 3B in the virus culture medium.
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Received: 25 May 2009
Published: 07 December 2009
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| Fund: Key Project of Chinese National Programs for Fundamental Research and Development |
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