Studies on expression of PGA gene in Saccharomyces cerevisiae

China Biotechnology

2008, Vol. 28

Issue (11): 32-35 DOI:

Studies on expression of PGA gene in Saccharomyces cerevisiae

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Abstract

In this study, the PGA gene of Bacillus megaterium 1.1741 was amplified by PCR, using primers designed on the base of PGA gene sequences of Bacillus megaterium in GenBank. Then the amplified PGA gene was cloned into pYES2 (amp+ ura+) vector, with T7lac promoter as the control. The constructed recombinant plasmid, named as pYES2-PGA, was transformed into Saccharomyces cerevisiae H158 (his- trp- leu- ura-) according to the LiAc/SSDNA/PEG method. PGA activity was detected in the culture liquid of recombinant Saccharomyces cerevisiae without penylacetic acid as the inducer, and the highest PGA activity was 0.75 U/ml. The sequence of PGA gene (present study) showed a high homology of 97.1%, 99.8% and 99.8% with three other strains of Bacillus megaterium L04471.1, U07682.1 and Z37542 in GenBank, respectively.

Key words： Bacillus megaterium PGA Saccharomyces cerevisiae gene expression

Received: 14 May 2008 Published: 20 April 2009

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	CHEN Wen-Ying
	DIAO Min
	WEI De-Jiang

Cite this article:

. Studies on expression of PGA gene in Saccharomyces cerevisiae. China Biotechnology, 2008, 28(11): 32-35.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I11/32

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