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Studies on Two-stage pH Control Strategy of Recombinant Cutinase Production |
Jian Chen |
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Abstract To enhance the yield and productivity of cutinase from recombinant Bacillus subtilis WSHB06-07, the effect of pH on cell growth and product formation was investigated. Based on specific cell growth rate and specific cutinase formation rate under different pH condition, a two stage pH control strategy was developed, in which pH was controlled at 7.5 for the first 4h and then shift to 6.5. By the utilization of this strategy, the yield of cutinase was significantly improved, the maximal cutinase activity and the productivity were 170 U/mL and 16.9 kU/(L·h), which were increased by 122.6% and 123.2%, respectively, compared to that from the condition of constant pH 7.5.
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Received: 31 January 2008
Published: 25 May 2008
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