|
|
The Determination and Control of Coproporphyrin Ⅲ in Vitamin B12 Fermentation by Pseudomonas denitrificans |
WANG Ze-jian, ZHAO Lin-lin, CHU Ju, ZHANG Ying-ping, ZHANG Si-liang |
State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China |
|
|
Abstract The accumulation of coproporphyrin Ⅲ,which is byproduct generated in vitamin B12 fermentation of Pseudomonas denitrificans,greatly affect the biosynthesis of vitamin B12 and the extraction in industrial production. An effective method for determining coproporphyrin Ⅲ concentration directly after suitable treatment of the fermentation broth. Furthermore,the influence of oxygen supply levels,carbon dioxide concentrations and pH on coproporphyrin Ⅲ biosynthesis were investigated,the optimal control strategy was implemented in 120 m3 industrial bioreactor. The results revealed that higher oxygen supply promoted the coproporphyrin Ⅲ accumulation,controlling the inlet carbon dioxide concentration at 8.6±0.8% and pH at 7.0±0.12 could greatly inhibit the coproporphyrin Ⅲ biosynthesis,and the vitamin B12 production increased for 15% than that of control.
|
Received: 31 March 2011
Published: 25 August 2011
|
|
|
|
[1] 罗伟,郝常明. 维生素B12的研究及其进展. 中国食品添加剂,2002,3:15-18.Luo W,Hao C M.China Food Additives,2002,3:15-18.
[2] Martens J H,Barg H,Warren M J,et al. Microbial production of vitamin B12. Appl Microbiol Biotechnol,2002,58:275-285.
[3] Scott A I,Roessner C A,Stolowich N J,et al. Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative ring contraction and insertion of the fourth methyl group. FEBS Lett,1993,331:105-108.
[4] Warren M J,Raux E,Schubert H L,et al. The biosynthesis of adenosylcobalamin(vitamin B12). Nat Prod Rep,2002,19:390-412.
[5] Li K T,Liu D H,Li Y L,et al. An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B12 by Pseudomonas denitrificans. Bioprocess and Biosystems Engineering,2008,31:605-610.
[6] Li K T,Liu D H,Li Y L,et al. Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding. Bioresource Technology,2008,99:8516-8520.
[7] Demain A L,White R F. Porphyrin overproduction by Pseudomonas denitrificans:essentiality of betaine and stimulation by ethionine. J Bacteriol,1971,107:456-460.
[8] Warren M J. Finding the final pieces of the vitamin B12 biosynthetic jigsaw. Proc Natl Acad Sci U S A,2006,103:4799-4800.
[9] Jermyn M A. Increasing the sensitivity of the anthrone method for carbohydrate. Anal Biochem,1975,68:332-335.
[10] Plakunov V K,Shelemekh O V. Mechanisms of oxygen regulation in microorganisms. Microbiology,2009,78:535-546.
[11] Wang Z J,Wang H Y,Li Y L,et al. Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process. Bioresource Technology,2010,101:2845-2852.
[12] Roessner C A,Santander P J,Scott A I,et al. Multiple biosynthetic pathways for vitamin B12:Variations on a central theme.In:Vitamins & Hormones.Litwack G,Begley T.San Diego:Academic Press,2001,61:267-297.
[13] Ferrentino G,Ferrari G,Poletto M,et al.,Microbial inactivation kinetics during high-pressure carbon dioxide treatment:nonlinear model for the combined effect of temperature and pressure in apple juice. J Food Sci,2008. 73(8):389-395.
[14] Wang D,Li Q,Li W L,et al. Improvement of succinate production by overexpression of a cyanobacterial carbonic anhydrase in Escherichia coli. Enzyme and Microbial Technology,2009,45:491-497.
[15] El-Sabbagh N,Harvey L M,McNeil B.Effects of dissolved carbon dioxide on growth,nutrient consumption,cephalosporin C synthesis and morphology of Acremonium chrysogenum in batch cultures. Enzyme and Microbial Technology,2008, 42:315-324.
[16] 王慧媛,王泽建,黄明志,等.CO2对脱氮假单胞菌发酵生产维生素B12的影响. 华东理工大学学报(自然科学版),2010,36:499-505. Wang H Y,Wang Z J,Huang M Z,et al. Journal of East China University of Science and Technology(natural science edition),2010,36:499-505.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|